Part:BBa_K1647002:Design

B0034+ho1+B0034+pcyA+B0010

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1564
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1117
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Used a strong RBS to produce high levels of expression of two enzymes for phycocyanobilin synthesis in E. Coli. Added a terminator for convenience.

Source

Assembled from parts in the previous distribution kits.